One-Photon Fluorescence Microscopy

One-photon fluorescence microscopy is an important biological and biomedical imaging technique. This chapter provides a comprehensive introduction of one-photon microscopy to help researchers maximize the effectiveness of their imaging experiments. This chapter first introduces fluorescence generation and the diffraction limit as background. It then outlines the basic operating principles of multiple one-photon microscopy configurations. Specific configurations include wide-field microscopy, light-field microscopy, confocal microscopy, light-sheet microscopy, and super-resolution microscopy. This chapter concludes by discussing multiple specific applications of one-photon fluorescence microscopy in neuroscience, matching the capabilities of the various microscope configurations with their role in obtaining novel information from biological samples.