RABBIT POLYMORPHONUCLEAR LEUKOCYTE MIGRATION IN VIVO IN RESPONSE TO LIPOPOLYSACCHARIDES FROM BACTEROIDES, FUSOBACTERIUM AND VEILLONELLA

Subcutaneously implanted chambers in rabbits were used for testing the migration of polymorphonuclear leukocytes in response to injected LPS isolated from strains of Bacteroides, Fusobacterium and Veillonella. A Salmonella LPS was used as reference endotoxin. No difference in chemotactic activity between the Veillonella LPS and LPS from Salmonella was found. Fusobacterium LPS showed insignificantly lower chemotactic capacity than the Salmonella LPS. The Bacteroides LPS were all significantly less chemotactic than the reference endotoxin. An insignificant correlation between the amount of exudate aspirated from the chambers 5 h after injection of the different LPS preparations and the number of leukocytes per μl of exudate was found.