化学
牛血清白蛋白
纳米颗粒
荧光
菁
检出限
共轭体系
血清白蛋白
核化学
色谱法
生物物理学
纳米技术
生物化学
有机化学
聚合物
材料科学
生物
量子力学
物理
作者
Lijun Xu,Guimei Jiang,Hong Chen,Zan Yue,Shanni Hong,Tingting Zhang,Yuanyuan Zhang,Renjun Pei
出处
期刊:Talanta
[Elsevier]
日期:2019-03-01
卷期号:194: 643-648
被引量:13
标识
DOI:10.1016/j.talanta.2018.10.094
摘要
Serum albumin has a wide range of applications in biochemical experiments and pharmaceutical field. We found that a cyanine dye, dimethylindole red (Dir), could selectively interact with bovine serum albumin (BSA). Dir exhibited very weak red fluorescence, while the fluorescence intensity at 630 nm was enhanced up to 130-fold upon noncovalently interacting with 30 µM BSA. Besides, Dir showed a highly selective response to BSA over human serum albumin (HSA). For the detection of BSA, a limit of detection as low as 23 nM was obtained. Then biocompatible Dir-BSA nanoparticles were prepared by the desolvation technique. The Dir-BSA nanoparticles possess excellent fluorescence properties with a quantum yield of 32%. Furthermore, folic acid as a targeting group was conjugated to Dir-BSA nanoparticles and these nanoparticles were characterized by TEM and laser particle analyzer, etc. Folic acid-modified Dir-BSA nanoparticles were successfully used for tumor cell-targeted imaging.
科研通智能强力驱动
Strongly Powered by AbleSci AI