纳米探针
检出限
材料科学
谷胱甘肽
DNA
生物物理学
生物医学中的光声成像
荧光团
分子成像
癌细胞
介孔二氧化硅
膜
癌症研究
光热治疗
体内
催化作用
小RNA
纳米颗粒
荧光寿命成像显微镜
纳米技术
癌症
化学
临床前影像学
荧光
介孔材料
生物化学
生物
色谱法
酶
物理
光学
遗传学
量子力学
作者
Kai Zhang,Xiangdan Meng,Zhou Yang,Yu Cao,Yaru Cheng,Dongdong Wang,Huiting Lu,Zhuojie Shi,Haifeng Dong,Xueji Zhang
标识
DOI:10.1002/adma.201807888
摘要
Herein, a cancer cell (MCF-7 cell) membrane-encapsulated dendritic mesoporous silica nanoparticle simultaneously functionalized with DNA-photoacoustic (DNA-PA) probes and glutathione (GSH)-responsive DNA fuel strands for PA imaging of tumor-related miRNA in living mice with signal amplification ability is developed. It is demonstrated that one target miRNA can trigger disassembly of multiple PA fluorophore probes from the quencher with the aid of GSH-responsive DNA fuel strands via the entropy-driven process, resulting remarkable amplified change of PA signal ratio. Using oncogenic miRNA-21 as a model, a linear relationship between miRNA-21 concentrations and PA ratio in a dynamic range from 10 × 10-12 m to 100 × 10-9 m and a limit of detection down to 11.69 × 10-12 m are established. The accurate PA signal observation related to miRNA-21s in the tumor area in living mice is demonstrated, and the PA signal ratio increases significantly via the injection of miRNA-21. It is anticipated that the catalytic ratiometric PA imaging system can be applied to an array of molecular detection in living system by rational detection probe design.
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