基因敲除
乳腺癌
互补DNA
信使核糖核酸
癌症研究
生物
乳腺肿瘤
克隆(编程)
癌
细胞培养
内科学
医学
癌症
基因
乳腺癌
遗传学
计算机科学
程序设计语言
作者
Yanyan Wang,Ning Sheng,Ying Xie,Sihan Chen,Jun Lü,Zifeng Zhang,Qun Shan,Dongmei Wu,Gui‐Hong Zheng,Mengqiu Li,Yuxin Zheng,Shao‐Hua Fan
摘要
Abstract The discovery of cysteine‐rich secretory protein 3 (CRISP3) has been made in human neutrophils for the first time. Cloning of the complementary DNA (cDNA) for CRISP3 was performed from a cDNA library of human bone marrow. In patients with mammary carcinoma, we found that lower expression of CRISP3 was connected to a significantly improved DFS (disease‐free survival) and OS (overall survival). Furthermore, the CRISP3 protein level was significantly associated with negative ANXA1 protein level. In addition, the heterogeneous expression of CRISP3 had been exhibited in diverse mammary carcinoma cells. A significant higher mRNA and the protein level of CRISP3 were seen in T‐47D as well as SK‐BR‐3 cells compared with those in other types of mammary carcinoma cells. Knockdown of CRISP3 in T‐47D or SK‐BR‐3 cells resulted in the weakened migration or invasion abilities. Furthermore, CRISP3 knockdown significantly inhibited the ERK1/2 MAPK signaling pathway in T‐47D or SK‐BR‐3 cells. Research results indicated that the lowering in the expression of CRISP3 is likely to serve as an unprecedented approach for the treatment of mammary carcinoma.
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