Qualitative and quantitative determination of active ingredients in Rukangshu capsules

Objective:To study qualitative and quantitative determination of some active ingredients, such as icariin, paeoniflorin and protocatechualdehyde, in Rukangshu capsules. Methods: karri-in,paeoniflorin and protocatechualdehyde were identified by TLC technique and the content of icariin and paeoniflorin were determined by HPLC method. Results: Icariin, paeoniflorin and protocatechualdehyde were clearly separated in the TLC chromatogram. The results of HPLC analysis for icariin were: a linear range of 92.4-3 326. 4ng, r = 0.999 9, an average recovery of 100.4 % with a RSD of 1.84 % and the corresponding data for paeoniflorin were: 93.0 - 3 348. Ong, r=0. 999 7, an average recovery of 100.3 % with a RSD of 1.46 % . Conclusion: The qualitative and quantitative methods established are capable to be used for quality control of Rukangshu capsules.