Study of Tissue Culture and Rapid Propagation of Rigghedan Ba Kos Strains

We take the leaves of Rigghedan Ba Kos strains as materials to conduct tissue culture technology research,including induction culture explanting subculture to adventitious buds,rooting culture to rootless seedling,in order to explore the optimal culture medium and culture condition.The results show that the best effect of induction adventitious buds on Rieger begonias leaves occurred in culture medium as MS+BA 1.0 mg/L+NAA 0.2 mg/L medium;subculture proliferation effect is better in culture medium as MS+BA 0.1 mg/L+NAA 0.01 mg/L;rooting induction training effect is better in culture medium as 1/2 MS +NAA 0.2 mg/L.As seedling height reaches 6 cm,transplant could be carried.After transplanting,highest survival rate could realize under the condition as maintaining more than 90% air humidity,temperature(25±3) ℃.