Glucosinolates composition and contents were evaluated taking 6 radish(Raphanus sativus L.)cultivars as experiment materials.The result indicates that there are great differences among total glucosinolates contents of different radish cultivars.'Jinghong No.3',an autumn-red radish,has the highest total glucosinolate content 480.9μmol·kg-1(FW),followed by'Jingyanshuiguoxingluobo' 318.8μmol·kg-1(FW).The lowest concent is found in'Mantanghong'213.9μmol·kg-1(FW),taking 44%of'Jinghong No.3'content.After analyzing glucosinolates composition,we find 5 types of radish glucosinolates,including 4-methylsulfinyl-3-butenyl-glucosinolates,4-hydroxyindol-3methyl-glucosinolates,4-methylthio-3-butenyl-glucosinolates,indol-3-methyl-glucosinolates and 4-methoxyindol-3-methyl-glucosinolates.The predominant glucosinolate in 6 radish varieties is 4-methylsulfinyl-3-butenyl-glucosinolate representing,taking about 70.5%-87.5%of the total amount.