成骨细胞
基因传递
小RNA
化学
骨质疏松症
体内
细胞生物学
药物输送
遗传增强
癌症研究
医学
生物化学
生物
体外
内科学
基因
生物技术
有机化学
作者
Ying Sun,Xiongzhen Ye,Mingxiang Cai,Xiangning Liu,Jia Xiao,Chenyang Zhang,Yayu Wang,Li Yang,Jiafan Liu,Shannai Li,Kang Chen,Bin Zhang,Qi Zhang,Zuolin Wang,Hongyu An,Xiaogang Wang
出处
期刊:ACS Nano
[American Chemical Society]
日期:2016-05-18
卷期号:10 (6): 5759-5768
被引量:118
标识
DOI:10.1021/acsnano.5b07828
摘要
Antiosteoporosis gene-based drug development strategies are presently focused on targeting osteoblasts to either suppress bone loss or increase bone mass. Although siRNA/microRNA-based gene therapy has enormous potential, it is severely limited by the lack of specific cell-targeting delivery systems. We report an osteoblast-targeting peptide (SDSSD) that selectively binds to osteoblasts via periostin. We developed SDSSD-modified polyurethane (PU) nanomicelles encapsulating siRNA/microRNA that delivers drugs to osteoblasts; the data showed that SDSSD-PU could selectively target not only bone-formation surfaces but also osteoblasts without overt toxicity or eliciting an immune response in vivo. We used the SDSSD-PU delivery system to deliver anti-miR-214 to osteoblasts and our results showed increased bone formation, improved bone microarchitecture, and increased bone mass in an ovariectomized osteoporosis mouse model. SDSSD-PU may be a useful osteoblast-targeting small nucleic acid delivery system that could be used as an anabolic strategy to treat osteoblast-induced bone diseases.
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