积雪草
色谱法
高效液相色谱法
重复性
指纹(计算)
化学
梯度洗脱
传统医学
医学
人工智能
计算机科学
作者
Qiang Lu,Wan-hong Li,Shi-qiang Hu
出处
期刊:Journal of Chinese medicinal materials
日期:2011-01-01
卷期号:34 (1): 46-50
被引量:3
摘要
Objective To study the HPLC fingerprints and establish a sensitive and specific method for controlling the quality of Centella asiatica. Methods HPLC gradient elution was applied for the fingerprints of Centella asiatica. All 16 samples are collected from different habitats of China. The columni was Alltech C18 column (250 mm x 4.6 mm, 5 microm), mobile phase was acetonitrile-water, flow rate was 1.0 ml/min, wavelength was 205 nm. Results The fingerprint of Centella asiatica was established, 16 samples of different areas of Centella asiatica were detected. There were 15 common peaks in the HPLC fingerprints of Centella asiatica. By comparison with the reference standards and using LC-ESI-MS(n) to corroborate the structure, 5-10 peaks were identified as madecassoside, asiaticoside, quercetin, kaemperol, madecassic acid and asiatic acid respectively. After calculating the similarity of the HPLC fingerprints of 16 habitants, the similarity of different habitats has been bad quite. Conclusion The method is accurate, reliable and good repeatability. This chromatographic fingerprint method can be used to controll the quality of Centella asiatica.
科研通智能强力驱动
Strongly Powered by AbleSci AI