The selection of Pleurotus eryngii、Stropharia rugosoannulata Farlow、Grifola Frondosa Mother Culture Medium and proper Mother Culture Medium nutritional grads more analyzed in the paper.The research shows that:of the five kinds of Mother Culture Medium(PDA Medium,PDA enriched Medium,wheat bran Medium,peptone dextrose agar Medium,apple juice Medium.)and the six nutritional grads(1~6),the mycelium of Pleurotus eryngii grows best on apple juice culture Medium at nutritional grads A3;the mycelium of Stropharia rugosoannulata Farlows grows best on wheat bran Medium at B5;the mycelium of Grifola Frondosa on PDA enriched culture Medium at D4.