Fluorescence Resonance Energy Transfer Between CdTe QDs and Rhodamine B with Its Application in The Determination of Lysozyme

The water-soluble CdTe was synthesized using thioglycolic acid as stabilizer.The fluorescence resonance energy transfer(FRET) between the CdTe QDs and Rhodamine B(RhB) in the presence of cetyltrimethylammonium bromide(CTAB) was characterized,in which the QDs with an emission wavelength of 530 nm was used as energy donor and rhodamine B as acceptor.Lysozyme content in lysozyme buccal tablets was determined by fluorescence resonance energy transfer.The results showed that the extent of fluorescence energy transfer quenching was linearly proportional to the concentration of lysozyme from 2×10-7 mol·L-1 to 8×10-6 mol·L-1 with a correlation coefficient of 0.991 0.The minimum detection concentration was 2×10-8 mol·L-1,RSD and the average recovery was 5.8% and 101%(n=5),respectively.