Isolation of Cellulase Producing Fungi and Its Effect on Degradation of Cellulose

Sixteen strains of large fungi collected in the field from Huaihua were isolated and purified.Congo red staining,the hydrolysis circle and colony diameter ratio(H/C value) were analysed on CMC solid medium to screen cellulase producing fungal strains.By determining the filter paper enzyme activity(FPA) in its supernatant of liquid fermentation culture,higher cellulase activity strain 01 were obtained ultimately.Factors including culture temperature,pH and incubating time affecting the production of cellulase had been studied by using the rice and CMC as carbon source.The results showed that the best culture temperature was 27℃,the optimal pH of culture medium and incubating time was 5.0 and 6d,respectively.Under the optimal condition,the FPA reached 580.0IU/mL.In this article,it showed that fungal 01 could been a candidate for future studying cellulase and feed production.