TRANSFORMATION OF BACILLUS AMYLOLIQUEFACIENS WITH GREEN FLUORESCENT PROTEIN GENE

The PSJ2 promoter cloned from Bacillus subtilis was spliced to gfp gene by overlap extension PCR.Thus the fused translational expression cassette PSJ2-gfp was constructed.After being digested by RcoR I and Pst I,PSJ2-gfp expression cassette was inserted into Bacillus subtilis vector pUS186 to give pUS186PGFP,which was then transformed into Bacillus amyloliquefaciens strain TB2 to give strain TB2GFP.The strain TB2GFP appeared weak green under blue light.The inhibiting effect of this strain on the growth of cucumerium,Fusarium oxyporum f.sp.,was not remarkably different from that of TB2.