Effects of Exposure Time to Gonadotrophin and Porcine Follicular Fluid on Maturation in Vitro of Porcine Oocytes

The aim of the present study was to investigate the effects of exposure time to gonadotrophin (FSH and LH) and porcine follicular fluid (pFF) on maturation in vitro of porcine oocytes.The results indicate that exposure of porcine cumulus-oocyte-complexes to gonadotrophin supplements for the first 2324 hours improves cumulus expansion but has no significant effect on oocyte nuclear maturation compared with that for 4648 hours (P0.05),and supplementation of a certain amount(10%) of porcine follicular fluid in maturation medium can enhance in vitro maturation of porcine oocytes, but excessive supplementation will relatively inhibit oocyte nuclear maturation process.