脱落酸
生物
渗透性休克
蛋白激酶A
激酶
磷酸化
蛋白激酶结构域
水稻
蔗糖
生物化学
突变体
细胞生物学
渗透调节
渗透浓度
基因表达
非生物胁迫
基因
作者
Yuhko Kobayashi,Shuhei Yamamoto,Hideyuki Minami,Yasuaki Kagaya,Tsukaho Hattori
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2004-05-01
卷期号:16 (5): 1163-1177
被引量:368
摘要
Abstract To date, a large number of sequences of protein kinases that belong to the sucrose nonfermenting1–related protein kinase2 (SnRK2) family are found in databases. However, only limited numbers of the family members have been characterized and implicated in abscisic acid (ABA) and hyperosmotic stress signaling. We identified 10 SnRK2 protein kinases encoded by the rice (Oryza sativa) genome. Each of the 10 members was expressed in cultured cell protoplasts, and its regulation was analyzed. Here, we demonstrate that all family members are activated by hyperosmotic stress and that three of them are also activated by ABA. Surprisingly, there were no members that were activated only by ABA. The activation was found to be regulated via phosphorylation. In addition to the functional distinction with respect to ABA regulation, dependence of activation on the hyperosmotic strength was different among the members. We show that the relatively diverged C-terminal domain is mainly responsible for this functional distinction, although the kinase domain also contributes to these differences. The results indicated that the SnRK2 protein kinase family has evolved specifically for hyperosmotic stress signaling and that individual members have acquired distinct regulatory properties, including ABA responsiveness by modifying the C-terminal domain.
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