Construction, Enzyme Response, and Substrate Capacity of a Hyaluronan–Cyclodextrin Supramolecular Assembly

A supramolecular assembly was constructed with a cationic cyclodextrin (EICD) and native hyaluronan (HA). The cationic carboxylic ester pendants on HA support hyaluronidase (HAase)-responsive sites and the EICD supports artificial carboxylic esterase responsive sites. Substrate-binding models were investigated by using environment-sensitive fluorescence probes 2-p-toluidino-6-naphthalenesulfoniate sodium (2,6-TNS) and thioflavin T (ThT). On a HA/EICD assembly, EICD was able to bind an anionic substrate and HA and EICD constructed the cationic substrate binding site together. This assembly could be used as a sequential dual-substrate carrier.