DNA断裂
精子
染色质
男科
生物
精子活力
卵胞浆内精子注射
精液
碎片(计算)
DNA
解剖
胚胎
遗传学
体外受精
医学
细胞凋亡
程序性细胞死亡
生态学
作者
Nino Guy Cassuto,A. Hazout,Ibrahim Hammoud,R. Balet,Dominique Bouret,Yona Barak,Sonia Jellad,Jean Marie Plouchart,J. Selva,Chadi Yazbeck
标识
DOI:10.1016/j.rbmo.2011.10.006
摘要
The utility of sperm DNA testing remains controversial. However, it may be helpful in couples with unexplained failures of multiple assisted reproductive techniques and/or recurrent abortions. This study analysed 10,400 spermatozoa of 26 patients for sperm-head morphology with high-magnification microscopy, DNA fragmentation and sperm chromatin decondensation. A significant negative correlation was demonstrated between sperm-parameters and abnormal sperm-head morphology as assessed by high magnification (score 0 according to this study’s classification): concentration (r = −0.41; P = 0.03), motility (r = −0.42; P = 0.03), morphology (r = −0.63; P = 0.0008). No correlation was found with DNA fragmentation. However, the sperm chromatin-decondensation rate of score-0 spermatozoa was twice as high as the controls (19.5% versus 10.1%; P < 0.0001). This observation suggests that score-0 spermatozoa should not be selected for intracytoplasmic sperm injection.We analysed 10,400 spermatozoa of 26 patients for sperm head morphology at high magnification, DNA fragmentation and sperm chromatin decondensation. We demonstrated a significant negative correlation between sperm parameters: concentration (r = –0.41; P = 0.03), motility (r = −0.42; P = 0.03), morphology (r = –0.63; P = 0.0008) and abnormal sperm head morphology as assessed by high magnification (score 0 according our classification). No correlation was found with DNA fragmentation. The sperm chromatin decondensation rate of score-0 spermatozoa was twice as high as than in controls (19.5% versus 10.1%; P < 0.0001). No significant relationship with sperm DNA fragmentation was observed. We suggest that high-magnification sperm selection could be an important step and a new tool for the clinician, who could decide to discard score-0 spermatozoa with a high risk of abnormal chromatin and select the best sperm cells for intracytoplasmic sperm injection.
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