Results. Starting from 2005 until 2009 a total of 559.476 cattle serum samples, from different villages across Macedonia, were collected and sent to the Faculty of Veterinary Medicine – Skopje, for testing for brucellosis. Seroprevalence in some epizootiological areas ranges from 0.1% up to 22.2%. According to the results, obtained with this study, the mean seroprevalence of cattle brucellosis in Macedonia for the last 5 years was estimated at 0.83% (ranging from 0.17% to 2.78%).