玻璃化
低温保存
再生医学
协议(科学)
胚胎干细胞
生物
计算机科学
干细胞
生化工程
细胞生物学
胚胎
男科
工程类
医学
病理
基因
替代医学
生物化学
作者
Boon Chin Heng,Lilia Kuleshova,Soren Müller Bested,Hua Liu,Tong Cao
摘要
hES (human embryonic stem) cells hold tremendous potential in the newly emerging field of regenerative medicine, in addition to being a useful tool in basic scientific research and for pharmacological and cytotoxicity screening. However, an essential prerequisite for the future widespread application of hES cells are the development of efficient cryopreservation protocols to facilitate their storage and transportation. This review summarizes the current state of progress in the field of hES cell cryopreservation, by critically examining and comparing the various cryopreservation protocols that have been developed. These can be classified into two categories: (1) conventional slow-cooling protocols and (2) vitrification protocols. Previously, the application of slow-cooling cryopreservation protocols to freely-suspended hES cell clumps yielded extremely dismal results. However, a recent study demonstrated that post-thaw survivability was markedly improved when slow-cooling protocols were applied instead to adherent hES colonies. Vitrification protocols have been shown to be much better than the standard slow-cooling protocol for the cryopreservation of freely suspended hES cell clumps. However, no study has yet attempted to apply vitrification protocols to adherent hES colonies. It must be noted that vitrification protocols are extremely labour-intensive and tedious to perform manually. Additionally, the use of cryostraws in vitrification protocols is unsuited for handling bulk quantities of hES cells, in addition to posing serious technical difficulties in developing machine automation for cryopreservation. These are some of the major challenges that have to be overcome if further progress is to be made in this field.
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