骨细胞
粒体自噬
自噬
细胞凋亡
缺氧(环境)
细胞生物学
化学
流式细胞术
成骨细胞
糖皮质激素
程序性细胞死亡
内分泌学
生物
分子生物学
生物化学
体外
氧气
有机化学
作者
Ke Xu,Chao Lü,Xiaoyu Ren,Jing Wang,Peng Xu,Yingang Zhang
摘要
Abstract Glucocorticoid (GC; dexamethasone, DEX) ‐induced osteonecrosis of the femoral head (GIOFH) is a challenging orthopedic disease, and its underlying mechanism remains not clear. This study exposed murine long bone osteocyte‐Y4 (MLO‐Y4) cells to DEX below normoxic or hypoxic circumstances and found that cell autophagy have been reduced. At the same time, flow cytometry analysis showed increased apoptosis, which was more pronounced in hypoxic environments. Recent research also claimed that GC induces osteoporosis after osteocyte apoptosis, and subsequent microfractures lead to ischemia and hypoxia of the femoral head, resulted in GIOFH. Presently, we found that both mitophagy‐related protein hypoxia‐inducible factor‐1α (HIF‐1α) and BNIP3 were up‐regulated in the hypoxic environment, and their expression was down‐regulated when exposed to DEX. Besides, we demonstrated that overexpressing HIF‐1α resisted DEX‐induced apoptosis in a hypoxic environment. Here, we demonstrated that overexpression of HIF‐1α, through its downstream marker BNIP3, reduced the suppression of DEX on mitophagy induced by hypoxia and protected bone cells from apoptosis. Also, these findings may provide a direction of the promising application for better GIOFH treatment shortly.
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