电化学发光
化学
纳米结构
检出限
纳米技术
猝灭(荧光)
电极
纳米颗粒
生物传感器
组合化学
材料科学
荧光
色谱法
物理化学
物理
量子力学
生物化学
作者
Jiekang Tian,Mei-Ling Zhao,Yu-Meng Song,Xia Zhong,Ruo Yuan,Ying Zhuo
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2021-10-05
卷期号:93 (41): 13928-13934
被引量:18
标识
DOI:10.1021/acs.analchem.1c02965
摘要
Herein, a new field-free and highly ordered spherical nucleic acid (SNA) nanostructure was self-assembled directly by ferrocene (Fc)-labeled DNA tweezers and DNA linkers based on the Watson-Crick base pairing rule, which was employed as an electrochemiluminescence (ECL) quenching switch with improved recognition efficiency due to the high local concentration of the ordered nanostructure. Moreover, with a collaborative strategy combined with the advantages of both self-accelerated approach and pore confinement-enhanced ECL effect, the mesoporous silica nanospheres (mSiO2 NSs) were prepared to be filled with rubrene (Rub) as ECL emitters and Pt nanoparticles (PtNPs) as coreaction accelerators (Rub-Pt@mSiO2 NSs), which demonstrated high ECL response in the aqueous media (dissolved O2 as coreactant). When the SNA nanostructure was immobilized on the Rub-Pt@mSiO2 NSs-modified electrode, it presented a "signal off" state owing to the quenching effect of the Fc molecules. As a proof of concept, the SNA-based ECL switch platform was applied in the detection of microRNA let-7b (let-7b). Impressively, in the presence of the target let-7b, a deconstruction of the SNA nanostructure was actuated, causing the Fc to leave the electrode surface and achieved an extremely high ECL recovery ("signal on" state). Hence, a sensitive determination for let-7b was realized with a low detection limit of 1.8 aM ranging from 10 aM to 1 nM by employing the Rub-Pt@mSiO2 NSs-based ECL platform combined with the target-triggered SNA deconstruction, which also offered an ingenious method for the further applications of biomarker analyses.
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