血管内皮生长因子
分子成像
糖尿病性视网膜病变
体内
离体
病理
临床前影像学
荧光寿命成像显微镜
医学
视网膜
眼科
血管内皮生长因子受体
视网膜
血管内皮生长因子A
荧光
癌症研究
生物
糖尿病
内分泌学
神经科学
光学
物理
生物技术
作者
Lu Zhang,Yue Ding,Xinjian Chen,Dehui Xiang,Fei Shi,Yanyun Chen,Shenshen Yan,Mengjie Zhang,Jie Tian,Sobha Sivaprasad,Yang Du,Zhi Yang,Bei Tian
摘要
Anti-vascular endothelial growth factor (anti-VEGF) therapy is effective for reducing the severity level of diabetic retinopathy (DR). However, it is difficult to determine the in vivo spatial and temporal expression of VEGF in the DR retina at an early stage. Here, we report a quantitatively fluorescence molecular imaging and image analysis method by creating a VEGF targeted fluorescence imaging probe, which can potentially detect and predict anti-VEGF treatment response. Moreover, the ex vivo multiscale fluorescence imaging demonstrated the spatial correlation between VEGF relative expression and vascular abnormalities in two and three dimensions. It revealed that VEGF was mainly abnormally expressed at the bifurcation of the microvessels, which advances the knowledge of the DR progression by molecular fluorescence imaging. Our study has the potential to achieve early detection of DR disease, provide more insight into understanding anti-VEGF treatment, and may help stratify patients based on the molecular imaging of retinal VEGF.
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