适体
检出限
MUC1号
化学
线性范围
纳米技术
离子
胶体金
离子通道
纳米颗粒
粘蛋白
色谱法
材料科学
分子生物学
生物化学
受体
有机化学
生物
作者
Michael Pan,Jiarong Cai,Si Li,Liguang Xu,Wei Ma,Chuanlai Xu,Hua Kuang
标识
DOI:10.1021/acs.analchem.0c04137
摘要
Detection of cancer markers is important for early diagnosis and timely treatment of cancer. In this study, we fabricated a tailorable gold nanofilm-anodized aluminum oxide (Au-AAO) ion channel through nanoparticle self-assembly and proposed a highly sensitive and selective Mucin 1 (MUC1) detection method. By engineering the optimal layers of the Au-AAO ion channel and encoding the aptamer between the interlayers, a highly controllable ion rectification phenomenon was observed. From this, the relationship between the rectification ratio (RR) and the concentration of MUC1 was established and the highly sensitive detection of MUC1 is achieved. We found that the aptamer-modified Au-AAO ion channel has a good linear range within the MUC1 concentration of 1–104 fg mL–1 and the limit of detection (LOD) was as low as 0.0364 fg mL–1 (0.0025 aM). Thus, this research opens a new horizon for fabricating multi-functional ion channels as well as developing ultrasensitive detection technologies.
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