质外体
谷胱甘肽还原酶
普通大麦
过氧化氢酶
生物
超氧化物歧化酶
过氧化物酶
抗氧化剂
丁香假单胞菌
谷胱甘肽
超氧化物
生物化学
植物
病菌
酶
谷胱甘肽过氧化物酶
微生物学
禾本科
细胞壁
作者
Hélène Vanacker,T. L. W. Carver,Christine H. Foyer
出处
期刊:Plant Physiology
[Oxford University Press]
日期:1998-07-01
卷期号:117 (3): 1103-1114
被引量:337
标识
DOI:10.1104/pp.117.3.1103
摘要
Leaves of two barley (Hordeum vulgare L.) isolines, Alg-R, which has the dominant Mla1 allele conferring hypersensitive race-specific resistance to avirulent races of Blumeria graminis, and Alg-S, which has the recessive mla1 allele for susceptibility to attack, were inoculated with B. graminis f. sp. hordei. Total leaf and apoplastic antioxidants were measured 24 h after inoculation when maximum numbers of attacked cells showed hypersensitive death in Alg-R. Cytoplasmic contamination of the apoplastic extracts, judged by the marker enzyme glucose-6-phosphate dehydrogenase, was very low (less than 2%) even in inoculated plants. Dehydroascorbate, glutathione, superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase were present in the apoplast. Inoculation had no effect on the total foliar ascorbate pool size or the redox state. The glutathione content of Alg-S leaves and apoplast decreased, whereas that of Alg-R leaves and apoplast increased after pathogen attack, but the redox state was unchanged in both cases. Large increases in foliar catalase activity were observed in Alg-S but not in Alg-R leaves. Pathogen-induced increases in the apoplastic antioxidant enzyme activities were observed. We conclude that sustained oxidation does not occur and that differential strategies of antioxidant response in Alg-S and Alg-R may contribute to pathogen sensitivity.
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