补语(音乐)
结构生物学
组分(热力学)
补体系统
重组DNA
计算生物学
样品制备
样本复杂性
化学
生物
生物系统
生物物理学
纳米技术
材料科学
生物化学
色谱法
计算机科学
基因
免疫学
物理
人工智能
抗体
热力学
表型
互补
作者
Alessandra Zarantonello,Sofia Mortensen,N.S. Laursen,Gregers Rom Andersen
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 249-264
被引量:3
标识
DOI:10.1007/978-1-0716-1016-9_22
摘要
Activated complement component C4 (C4b) is the nonenzymatic component of the classical pathway (CP) convertases of the complement system. Preparation of C4 and C4b samples suitable for structural biology studies is challenging due to low yields and complexity of recombinant C4 production protocols reported so far and heterogeneity of C4 in native sources. Here we present a purification protocol for human C4 and describe sample preparation methods for structural investigation of C4 and its complexes by crystallography, small angle X-ray scattering, and electron microscopy.
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