生物传感器
检出限
材料科学
乳腺癌
癌细胞
循环肿瘤细胞
生物医学工程
光纤布拉格光栅
癌症
纳米技术
光电子学
医学
化学
色谱法
内科学
转移
波长
作者
Xiaoyong Chen,Pin Xu,Wen‐Wei Lin,Jin Jang,Haiyan Qu,Xuehao Hu,Jinghua Sun,Yukun Cui
摘要
The detection of circulating tumor cells (CTCs) still faces a huge challenge partially because of low abundance of CTCs (1-10 cells/mL). In this work, a plasmonic titled fiber Bragg grating biosensor is proposed for detection of breast cancer cells. The biosensor is made by an 18° TFBG with a 50 nm-thick gold nanofilm coating over the surface of the fiber, further immobilized with a specific antibody against GPR30, which is a membrane receptor expressed in many breast cancers, serving as bait. In vitro tests have confirmed that the proposed biosensor can detect breast cancer cells in concentration of 5 cells/mL within 20 minutes and has good linearity in the range of 5–1000 cells/mL, which has met the requirement of CTC detection in real conditions. Furthermore, theoretical analysis based on the experimental results shows that the limit of detection can even reach single-cell level. Our proposed biosensor has a simple structure, is easy to manufacture, is of small size, and has a good performance, making it a good choice for real-time, label-free, and milliliter-volume detection of cancer cells in future.
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