表面等离子共振
溶菌酶
乙二醇
聚合物
分子
材料科学
生物传感器
共振(粒子物理)
生物系统
化学物理
曲面(拓扑)
纳米技术
化学
物理
纳米颗粒
有机化学
原子物理学
数学
生物
生物化学
复合材料
几何学
作者
Justas Svirelis,John Andersson,Anna Stradner,Andreas Dahlin
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2022-03-17
卷期号:7 (4): 1175-1182
被引量:2
标识
DOI:10.1021/acssensors.2c00273
摘要
Surface plasmon resonance is a very well-established surface sensitive technique for label-free analysis of biomolecular interactions, generating thousands of publications each year. An inconvenient effect that complicates interpretation of SPR results is the "bulk response" from molecules in solution, which generate signals without really binding to the surface. Here we present a physical model for determining the bulk response contribution and verify its accuracy. Our method does not require a reference channel or a separate surface region. We show that proper subtraction of the bulk response reveals an interaction between poly(ethylene glycol) brushes and the protein lysozyme at physiological conditions. Importantly, we also show that the bulk response correction method implemented in commercial instruments is not generally accurate. Using our method, the equilibrium affinity between polymer and protein is determined to be KD = 200 μM. One reason for the weak affinity is that the interaction is relatively short-lived (1/koff < 30 s). Furthermore, we show that the bulk response correction also reveals the dynamics of self-interactions between lysozyme molecules on surfaces. Besides providing new insights on important biomolecular interactions, our method can be widely applied to improve the accuracy of SPR data generated by instruments worldwide.
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