化学
电容感应
周转时间
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
检出限
电容
微流控
色谱法
2019年冠状病毒病(COVID-19)
炸薯条
微流控芯片
电极
线性范围
纳米技术
计算机科学
医学
电信
材料科学
疾病
病理
物理化学
传染病(医学专业)
操作系统
作者
Haochen Qi,Zhiwen Hu,Zhongliang Yang,Jian Zhang,Cheng Cheng,Cheng Cheng,Chunchang Wang,Lei Zheng
标识
DOI:10.1021/acs.analchem.1c04296
摘要
The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has lasted for almost 2 years. Stemming its spread has posed severe challenges for clinical virus detection. A long turnaround time, complicated operation, and low accuracy have become bottlenecks in developing detection techniques. Adopting a direct antigen detection strategy, we developed a fast-responding and quantitative capacitive aptasensor for ultratrace nucleocapsid protein detection based on a low-cost microelectrode array (MEA) chip. Employing the solid-liquid interface capacitance with a sensitivity of picofarad level, the tiny change on the MEA surface can be definitively detected. As a result, the limit of detection reaches an ultralow level of femtogram per milliliter in different matrices. Integrated with efficient microfluidic enrichment, the response time of this sensor from the sample to the result is shortened to 15 s, completely meeting the real-time detection demand. Moreover, the wide linear range of the sensor is from 10-5 to 10-2 ng/mL, and a high selectivity of 6369:1 is achieved. After application and evaluation in different environmental and body fluid matrices, this sensor and the detection method have proved to be a label-free, real-time, easy-to-operate, and specific strategy for SARS-CoV-2 screening and diagnosis.
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