血管生长素
等电点
绒毛尿囊膜
氨基酸
体内
生物化学
化学
细胞培养
等电聚焦
脯氨酸
生物
内皮抑素
分子生物学
血管生成
体外
癌症研究
酶
生物技术
遗传学
作者
James W. Fett,Daniël J. Strydom,Roy R. Lobb,Edward M. Alderman,J. L. Bethune,James Riordan,Bert L. Vallée
出处
期刊:Biochemistry
[American Chemical Society]
日期:1985-09-01
卷期号:24 (20): 5480-5486
被引量:877
摘要
The first human tumor derived protein with in vivo angiogenic activity to be obtained in pure form has been isolated from serum-free supernatants of an established human adenocarcinoma cell line (HT-29) and named angiogenin. It was purified by cation-exchange and reversed-phase high-performance liquid chromatography; the yield was approximately 0.5 microgram/L of medium. Biological activity of angiogenin was monitored throughout purification by using the chick embryo chorioallantoic membrane assay. Statistical evaluation demonstrates that it displays activity in this system with as little as 35 fmol per egg. Moreover, only 3.5 pmol is required to induce extensive blood vessel growth in the rabbit cornea. The amino acid composition of this basic (isoelectric point greater than 9.5), single-chain protein of molecular weight approximately 14 400 has been determined. The amino terminus is blocked, and the carboxyl-terminal residue is proline.
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