化学
小分子
蛋白质-蛋白质相互作用
生物物理学
组合化学
分子
生物分子
生物化学
计算生物学
靶蛋白
细胞生物学
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2007-03-01
卷期号:2007 (3): pdb.prot4631-pdb.prot4631
被引量:1
摘要
INTRODUCTIONTo detect protein interactions with small molecules, a protein microarray is incubated with a fluorescently labeled compound, the array is washed, and stable interactions are identified by scanning the slide for fluorescence. It is often advantageous to label the small molecules indirectly, by covalently linking them to a previously labeled carrier molecule such as BSA. This protocol describes the labeling of BSA with Cy3 or Cy5, the linkage of the small molecule to the labeled BSA, and the probing of protein microarrays with the fluorescently labeled conjugate.
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