适体
检出限
虎耳草毒素
亚甲蓝
生物传感器
胶体金
化学
电化学
选择性
纳米技术
组合化学
电极
色谱法
材料科学
纳米颗粒
分子生物学
物理化学
生物
催化作用
光催化
生物化学
毒素
作者
Weixian Zheng,Xinyu Liu,Qianwen Li,Zuju Shu,Zhongbo Li,Lijun Zhang
出处
期刊:RSC Advances
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:12 (37): 23801-23807
被引量:4
摘要
In this study, a simple electrochemical aptasensor for saxitoxin (STX) detection was developed based on a short anti-STX aptamer with a methylene blue (MB) incorporated at the 3'-end. The aptamer was immobilized on the surface of gold electrode and served as the recognition element. The aptasensor was successfully used as a highly selective and sensitive biosensor for STX detection because of the selection of aptamer along with the excellent electrocatalytical properties of MB. With the increase of the dynamic concentration of STX from 1 nM to 1 μM, the aptasensor exhibited a sensitivity with a peak current change of 2.92 μA per decade and a low detection limit of 1 nM. In addition, the aptasensor demonstrated excellent selectivity to STX, which prevented the interferences from neo-STX, okadaic acid and common metal ions. Using the proposed principle, several other aptasensors with improved performance can be constructed via the modification of the electrochemical sensor with appropriate aptamers.
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