Three kinds of agricultural residues — broad bean straw, wheat straw, and corn cobs — were tested as substrates for Pleurotus ostreatus cultivation. The best yield of fruit bodies (up to 79%) was achieved using broad bean straw. Corn cobs yielded the least (34.5%). Wheat straw showed the greatest material loss through respiration, making it the least efficient. Broad bean straw allowed for up to 1.94 g of fruit bodies per 1 g of decomposed substrate, compared to 0.95 g for wheat straw. It also had the highest nitrogen content (1.02%).