Multicolor emission-based nitrogen, sulfur and boron co-doped photoluminescent carbon dots for sequential sensing of Fe3+ and cysteine: RGB color sensor and live cell imaging

Herein, a simple hydrothermal synthesis is used to prepare multiple heteroatom-doped photoluminescent carbon dots (CDs) from thiourea (N and S source) and boric acid (B source) as precursors. The optical and physicochemical properties of the as-synthesized NSB-CDs were studied using UV–Vis, photoluminescence, TEM, FT-IR, XRD, Raman, and XPS analyses. The NSB-CDs exhibited excellent stability, high photostability, pH, and ionic strength tolerance; they retained their excellent stability independent of excitation. The NSB-CDs featured small sizes of approximately 3.2 ± 0.4 nm (range: 2.0–5.0 nm) as evidenced using TEM measurements. The NSB-CDs were used as a photoluminescent sensing platform to detect Fe3+ as well as cysteine (Cys) molecules. The competitive binding of Cys to Fe3+ resulted in NSB-CDs that retained their photoluminescence. For the rapid identification and quantification of Fe3+ and Cys, NSB-CDs were developed as a “switch-on” dual-function sensing platform. The linear detection range of Fe3+ was 0–20 μM (limit of detection [LOD]: 54.4 nM) and that of Cys was 0–50 μM (LOD: 4.9 nM). We also introduced a smartphone RGB analysis method for detecting low-concentration solutions based on digital images. The NSB-CDs showed no toxicity at 100 μg/mL. Photoluminescent probes for multicolor live-cell imaging can be used with NSB-CDs at this concentration, suggesting that NSB-CDs may be promising photoluminescent probes.