Novel Insights for α-amylase Improvement: Leveraging Amylopectin into Accurate Molecular Docking and Mutant Selection

, respectively─demonstrated α-amylase activity improvements of 101.6 and 89.7%, respectively, compared to their wild-type counterparts. This study underscores the importance of using amylopectin for precise molecular docking analyses, providing a new strategy for optimizing α-amylase and advancing industrial enzyme applications.