Natural resources, quantification, microbial bioconversion, and bioactivities of vitamin B12 for vegetarian diet

Vitamin B 12 is a water-soluble vitamin with a complex chemical structure. It can participate in the synthesis and repair of DNA in the human body and plays an important role in regulating the nervous system. The deficiency of vitamin B 12 will lead to megaloblastic anemia and neuropathy . Traditionally, animal foods have been the main dietary source of vitamin B 12 . However, this review points to certain plant sources (such as algae, mushrooms, fermented vegetables , and fermented beans) as viable vitamin B 12 supplements for vegetarians. These sources validate our initial hypothesis that a plant-based diet can adequately provide essential nutrients previously thought to be available only through animal products. In terms of quantification, since the content of vitamin B 12 in food samples is low and is easily interfered by impurities, highly sensitive and specific analytical methods are used for the quantification of vitamin B 12 . The findings from this review could be instrumental in developing fortified plant-based foods that could prevent B 12 deficiency in vegetarians and vegans, thereby broadening the scope of nutritional options available to those on plant-based diets.