Preparation of mannan-oligosaccharides magnetic microspheres

The mannan-oligosaccharides magnetic microspheres were prepared with MOS and Fe3O4.Using average particle size and magnetic responsiveness as indicators,the effect of different factors on preparation of mannan-oligosaccharides magnetic microspheres were researched by single-factors tests,the complex was detected by IR and SEM to check its symbol.The synthetic conditions of mannan-oligosaccharides magnetic microspheres were optimized through orthogonal test and the optimum experimental conditions are as follows: concentration of MOS was 0.01 g/mL,reaction time was 60 min,cross-linking temperature was 60 ℃ and the volume of ECH was 8 mL.