Effects of different methods of elution on extraction quality of cephalosporin C

Objective To investigate the effects of different methods of elution on the extraction quality of cephalosporin C.Methods Cephalosporin C was absorbed when the fermentation filtrate of cephalosporin C was passed through a resin column of macro-porous adsorption resin XAD-16,and eluted by uniformity elution method and gradient elution method respectively.Results When gradient elution method was adopted,the purity of cephalosporin C in the eluent was 3.23% higher and the recovery rate of cephalosporin C in the eluent was 2.3% higher than that of uniformity elution method.Conclusions Gradient elution method is worthy of widely use in cephalosporin C production for its advantages compared with uniformity elution method.