计算生物学
剧目
噬菌体展示
利用
单域抗体
表位
抗体库
生物
抗体
免疫系统
分离(微生物学)
领域(数学分析)
计算机科学
生物信息学
免疫学
数学分析
物理
计算机安全
数学
声学
作者
M. Müller,Ronan O’Dwyer,Marina Kovaleva,Fiona M. Rudkin,Helen Dooley,Caroline J. Barelle
出处
期刊:Methods in molecular biology
日期:2012-01-01
卷期号:: 177-194
被引量:28
标识
DOI:10.1007/978-1-61779-974-7_9
摘要
The drive to exploit novel targets and biological pathways has lead to the expansion of classical antibody research into innovative fragment adaptations and novel scaffolds. The hope being that alternative or cryptic epitopes may be targeted, tissue inaccessibility may be overcome, and easier engineering options will facilitate multivalent, multi-targeting approaches. To this end, we have been isolating shark single domains to gain a greater understanding of their potential as therapeutic agents. Their unique shape, small size, inherent stability, and simple molecular architecture make them attractive candidates from a drug discovery perspective. Here we describe protocols to capture the immune repertoire of an immunized shark species and to build and select via phage-display target-specific IgNAR variable domains (VNARs).
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