Phosphorylation and citration of normal corn starch by dry heating with phytic acid and citric acid

Normal corn starch was subjected to dry heating in the presence of phytic acid (PA, 2 %, starch basis) and citric acid (CA, 5 % and 10 %) for modification. Dual treatment with PA and CA induced structural and physicochemical changes in normal corn starch. Phosphorus concentration, degree of substitution, FTIR, and 31P NMR analyses confirmed esterification of starch by dry heating with PA and CA. Both phosphorylation and citration by esterification with PA and CA were observed in PA + 5CA starch, but high CA concentration inhibited covalent interaction between PA and starch in PA + 10CA starch. The degree of phosphorylation and citration resulted in different physicochemical properties in starch treated with PA and CA. The treatment with only PA did not change the crystalline regions of PA starch, but CA treatment induced the disruption of the crystalline structure of PA + 5CA and PA + 10CA starch. PA starch showed high solubility (46.41 %) and transmittance (90.51 %), but dual treatment of PA and CA induced significant decrease in solubility (3.23 %) and transmittance (2.18 %) of PA + 10CA starch. CA treatment increased the fraction of resistant starch in non-cooked (72.44 %) and cooked PA + 10CA starch (42.76 %). Therefore, dual treatment with PA and CA had potential to control physicochemical and functional properties of starch by phosphorylation and citration of starch.