群体特异性抗原
核糖核酸
细胞生物学
信使核糖核酸
支架蛋白
病毒
膜
生物
化学
基因
病毒学
生物化学
信号转导
作者
Yachen Ying,Yantao Yang,Antony K. Chen
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2024-02-23
卷期号:10 (8)
标识
DOI:10.1126/sciadv.adk8297
摘要
HIV-1 Gag proteins can multimerize upon the viral genomic RNA or multiple random cellular messenger RNAs to form a virus particle or a virus-like particle, respectively. To date, whether the two types of particles form via the same Gag multimerization process has remained unclarified. Using photoactivated localization microscopy to illuminate Gag organizations and dynamics at the nanoscale, here, we showed that genomic RNA mediates Gag multimerization in a more cluster-centric, cooperative, and spatiotemporally coordinated fashion, with the ability to drive dense Gag clustering dependent on its ability to act as a long-stranded scaffold not easily attainable by cellular messenger RNAs. These differences in Gag multimerization were further shown to affect downstream selective protein sorting into HIV membranes, indicating that the choice of RNA for packaging can modulate viral membrane compositions. These findings should advance the understanding of HIV assembly and further benefit the development of virus-like particle-based therapeutics.
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