归巢(生物学)
数据库管理
脱钙骨基质
间充质干细胞
血管生成
干细胞
细胞生物学
骨愈合
CD90型
移植
免疫系统
间质细胞
免疫学
医学
病理
癌症研究
生物
解剖
材料科学
内科学
川地34
生态学
放大器
光电子学
CMOS芯片
作者
Qing‐Jiang Pan,Pei Zhang,Fengxia Xue,Jingxuan Zhang,Zhenlin Fan,Z. Chang,Zhiqing Liang,Guangdong Zhou,Wenjie Ren
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2023-12-27
标识
DOI:10.1021/acsbiomaterials.3c01331
摘要
Immunoregulatory and vascularized microenvironments play an important role in bone regeneration; however, the precise regulation for vascularization and inflammatory reactions remains elusive during bone repair. In this study, by means of subcutaneous preimplantation, we successfully constructed demineralized bone matrix (DBM) grafts with immunoregulatory and vascularized microenvironments. According to the current results, at the early time points (days 1 and 3), subcutaneously implanted DBM grafts recruited a large number of pro-inflammatory M1 macrophages with positive expression of CD68 and iNOS, while at the later time points (days 7 and 14), these inflammatory cells gradually subsided, accompanying increased presence of anti-inflammatory M2 macrophages with positive expression of CD206 and Arg-1, indicating a gradually enhanced anti-inflammatory microenvironment. At the same time, the gradually increased angiogenesis was observed in the DBM grafts with implantation time. In addition, the positive cells of CD105, CD73, and CD90 were observed in the inner region of the DBM grafts, implying the homing of mesenchymal stem cells. The repair results of cranial bone defects in a rat model further confirmed that the subcutaneous DBM xenografts at 7 days significantly improved bone regeneration. In summary, we developed a simple and novel strategy for bone regeneration mediated by anti-inflammatory microenvironment, prevascularization, and endogenous stem cell homing.
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