生物
卵巢癌
蛋白质表达
化学
转移
癌症研究
下调和上调
细胞生物学
癌症
基因
生物化学
遗传学
作者
Yaqian Shi,Xueyou Xiong,Yu Sun,Zhe Geng,Xiyi Chen,Xiaomin Cui,Juan Lv,Lili Ge,Xuemei Jia,Juan Xu
标识
DOI:10.1096/fj.202202145rrr
摘要
Abstract Ovarian cancer (OC) is the second leading cause of gynecological cancer‐related death in women worldwide. N6‐methyladenosine (m 6 A) is the most abundant internal modification in eukaryotic RNA. Human insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2), an m 6 A reader, can enhance mRNA stability and promote translation by recognizing m 6 A modifications. Its tumor‐promoting effects have been demonstrated in several cancers. However, the roles of m 6 A modification and IGF2BP2 in OC remain unclear. Here, by using methylated RNA immunoprecipitation sequencing, we demonstrated that there is widespread dysregulation of m 6 A modification in OC tissues. The m 6 A modification and the mRNA and protein levels of IGF2BP2 were significantly elevated in OC. Overexpression of IGF2BP2 facilitated OC cell proliferation, migration, and invasion in vitro and accelerated tumor growth and metastasis in vivo. While IGF2BP2‐knockdown showed the opposite effect. Mechanistically, we identified cytoskeleton‐associated protein 2‐like (CKAP2L) as a target of IGF2BP2. IGF2BP2 promoted CKAP2L translation dependent on m 6 A modification, rather than affecting mRNA and protein stability. Overexpression of CKAP2L rescued the tumor‐suppressive effect of IGF2BP2 knockdown in OC cells. In conclusion, this study revealed the potential role of IGF2BP2 in tumor progression, at least partially via promoting the translation of CKAP2L in an m 6 A‐dependent manner.
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