Comparative Evaluation of a Disinfectant Formulation Comprising Hydrogen Peroxide, Peracetic Acid, and Acetic Acid Against Aspergillus niger, Escherichia coli, and Staphylococcus aureus on Various Surfaces in Comparison to Other Disinfectants

This study investigates the effectiveness of a disinfectant formulation comprising acetic acid, hydrogen peroxide, and peracetic acid (AAHPA) against Aspergillus niger , Escherichia coli , and Staphylococcus aureus on petri plates, smooth surfaces, and rough surfaces. Comparative analysis with conventional disinfectants such as 70% isopropyl alcohol, 1.5% chlorhexidine gluconate, 10% sodium hypochlorite, and 0.2% hydrogen peroxide reveal AAHPA’s superior performance, achieving significantly higher log reduction ( p < 0.05) and percentage reduction ( p < 0.01) against A. niger in 5 min on petri plates. On smooth and rough surfaces, AAHPA exhibits exceptional efficacy, demonstrating the highest log and percentage reduction against A. niger in 0.5, 1, and 5 min, surpassing other disinfectants. AAHPA shows the highest bacterial decline against E. coli and S. aureus , followed by 0.2% hydrogen peroxide. In some cases, 0.2% hydrogen peroxide was slightly more effective than AAHPA against E. coli and S. aureus in 5 min. Time‐dependent analysis of log reduction trends emphasizes AAHPA’s rapid and consistent effectiveness, particularly in 0.5 min, positioning it as a promising disinfectant formulation with broad‐spectrum efficacy across diverse surfaces.