间充质干细胞
微泡
炎症
间质细胞
外体
免疫学
医学
干细胞
细胞
癌症研究
细胞生物学
生物
小RNA
病理
基因
生物化学
遗传学
作者
Hector Katifelis,Eirini Filidou,Adriana Psaraki,Farinta Yakoub,Maria G. Roubelakis,Gesthimani Tarapatzi,Stergios Vradelis,Giorgos Bamias,George Kolios,Maria Gazouli
出处
期刊:Biomedicines
[Multidisciplinary Digital Publishing Institute]
日期:2022-09-21
卷期号:10 (10): 2357-2357
被引量:12
标识
DOI:10.3390/biomedicines10102357
摘要
Inflammatory Bowel Diseases (IBDs) are characterized by chronic relapsing inflammation of the gastrointestinal tract. The mesenchymal stem/stromal cell-derived secretome and secreted extracellular vesicles may offer novel therapeutic opportunities in patients with IBD. Thus, exosomes may be utilized as a novel cell-free approach for IBD therapy. The aim of our study was to examine the possible anti-inflammatory effects of secretome/exosomes on an IBD-relevant, in vitro model of LPS-induced inflammation in human intestinal SubEpithelial MyoFibroblasts (SEMFs). The tested CM (Conditioned Media)/exosomes derived from a specific population of second-trimester amniotic fluid mesenchymal stem/stromal cells, the spindle-shaped amniotic fluid MSCs (SS-AF-MSCs), and specifically, their secreted exosomes could be utilized as a novel cell-free approach for IBD therapy. Therefore, we studied the effect of SS-AF-MSCs CM and exosomes on LPS-induced inflammation in SEMF cells. SS-AF-MSCs CM and exosomes were collected, concentrated, and then delivered into the cell cultures. Administration of both secretome and exosomes derived from SS-AF-MSCs reduced the severity of LPS-induced inflammation. Specifically, IL-1β, IL-6, TNF-α, and TLR-4 mRNA expression was decreased, while the anti-inflammatory IL-10 was elevated. Our results were also verified at the protein level, as secretion of IL-1β was significantly reduced. Overall, our results highlight a cell-free and anti-inflammatory therapeutic agent for potential use in IBD therapy.
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