生物
生物病虫害防治
菌丝体
真菌
爆发性疾病
拉伤
抗真菌
细菌
芽孢杆菌(形态)
植物病害
枯草芽孢杆菌
微生物学
生物技术
植物
生物化学
遗传学
解剖
基因
作者
Rongsheng Zhang,Faguo Wang,Zhongqiang Qi,Junqing Qiao,Yan Du,Junjie Yu,Mina Yu,Dong Liang,Tianqiao Song,Pan-Xia Yan,Huijuan Cao,Hao Zhang,Yongfeng Liu
标识
DOI:10.1016/j.biocontrol.2022.105001
摘要
Rice blast disease, caused by the plant pathogenic fungus Magnaporthe oryzae, severely constrains rice production worldwide. Using plant-associated microorganisms, biological control of rice blast is an efficient and environmentally friendly approach to disease management. Bacillus bacteria produce a broad array of antifungal lipopeptides, making them effective biological control agents. In a previous study, Bacillus velezensis strain Jt84 exhibited biocontrol activity against rice blast disease, and the antifungal compounds it produces were isolated by acid precipitation of cell-free supernatants extracted with methanol were lipopeptides. Lipopeptides showed a stronger inhibitory effect against M. oryzae and induced abnormal mycelial morphology. Further analysis using PCR and HPLC-ESI-MS confirmed the presence of surfactins, iturins, and fengycins. The results exhibited that iturins play an essential role in the antifungal activity against M. oryzae and confirmed these results through mutagenesis experiments used to suppress iturins biosynthesis. In conclusion, B. velezensis Jt84 significantly reduced the incidence of rice blast disease at the seedling stage, while B. velezensis Jt84ΔituB strain markedly reduced disease biocontrol effect, indicated that iturins produced by B. velezensis Jt84 strain play a key role in the biocontrol of rice blast disease.
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