血管生成
伤口愈合
炎症
血管内皮生长因子
新生血管
细胞外基质
治疗性血管生成
间质细胞
化学
再生(生物学)
药理学
细胞生物学
医学
脚手架
癌症研究
免疫学
血管内皮生长因子受体
生物医学工程
生物
生物化学
作者
Ge Long,Dingyang Liu,Xi He,Yeyu Shen,Yannan Zhao,Xun Hou,Bing Chen,Wen Ouyang,Jianwu Dai,Xing Li
出处
期刊:Biomaterials Science
[The Royal Society of Chemistry]
日期:2020-01-01
卷期号:8 (22): 6337-6349
被引量:45
摘要
Chronic diabetic wounds, which are associated with persistent inflammation and impaired angiogenesis, occur frequently in diabetic patients. Some studies have shown that separate administration of vascular endothelial growth factor (VEGF) or stromal cell derived factor 1α (SDF-1α) exhibited a therapeutic effect in promoting angiogenesis in the wound healing process. In this study, a collagen membrane is prepared as a drug delivery scaffold to investigate whether combined administration of SDF-1α and VEGF has a synergistic therapeutic effect on diabetic wound healing. We specifically fused a collagen-binding domain (CBD) with SDF-1α and VEGF separately, and sustained release of the two recombinant proteins from the collagen scaffold is successfully observed. Meanwhile, when a CBD-VEGF and CBD-SDF-1α co-modified scaffold is implanted in a diabetic rat skin wound model, it not only shows a synergistic effect in facilitating angiogenesis but also reduces inflammation in the short-term. Moreover, long-term results reveal that the co-modified scaffold is also able to enhance rapid wound healing, promote blood vessel regeneration, and assist cell proliferation, re-epithelialization and extracellular matrix accumulation. Taken together, our study indicates that the CBD-VEGF and CBD-SDF-1α co-modified scaffold helps in quick recovery from diabetic wounds by coordinating angiogenesis and inflammation.
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