Ratiometric fluorescence nanoprobe for monitoring of intracellular temperature and tyrosine based on a dual emissive carbon dots/gold nanohybrid

A novel dual-emission nitrogen doped carbon dots/gold nanohybrid (NCDs-Au) was designed for specific and sensitive ratiometric detection of intracellular temperature and tyrosine. In this probe, a reductive NCDs was successfully prepared with the use of natural biomass Dendrobium officinale as precursor. The new prepared NCDs acted as both reducers and stabilizers to synthesize a novel NCDs-Au nanohybrid by a facile one-step procedure along with a quantum yield of 14.3%. The prepared nanoprobe showed characteristic fluorescence emissions of NCDs and Au NCs with single-wavelength excitation. Notably, the nanoprobe shows an interesting wavelength-dependent dual response to temperature (448 nm) and tyrosine (660 nm), enabling the two targets to be detected proportionally. As an effective temperature sensor, the nanoprobe exhibited good temperature-dependent fluorescence with a sensational linear response from 5 to 75 °C. In addition, the sensor has a linear response toward tyrosine in the range of 0.5–175 μM with a detection limit of 0.19 μM. Moreover, the fluorescent nanoprobe was successfully applied to ratiometricly monitor the variation of temperature or tyrosine level in cells because of the low cytotoxicity, chemical stability and excellent fluorescence properties. These results suggested that the nanoprobe here has provided the possibility for rapidly biosensing with the acceptable selectivity and sensitivity. • The NCDs prepared with natural biomass Dendrobium officinale were used as a reducer and stabilizer. • The NCDs-Au possesses two emission peaks at 448 nm (blue) and 660 nm (red) under a single excitation wavelength. • The dual-emission ratiometric fluorescence nanoprobe was employed for sensing of temperature and tyrosine. • The nanoprobe was successfully applied to ratiometricly monitor the variation of temperature and tyrosine in live cells.