牛血清白蛋白
化学
胎牛血清
生物物理学
蛋白质降解
细胞生物学
细胞毒性
牛乳头状瘤病毒
日冕(行星地质学)
纳米毒理学
内化
降级(电信)
细胞
毒性
体外
生物化学
生物
基因
天体生物学
基因组
有机化学
电信
计算机科学
维纳斯
作者
Tan Ya,Xiangyu Zhu,Di Wu,Erqun Song,Yang Song
标识
DOI:10.1021/acs.est.0c04097
摘要
The adverse biological and ecological consequences of plastic debris have become a serious problem worldwide. Evidences have uncovered the accumulation of nanoplastics (NPs) in organisms. In a complex biological environment, proteins are prone to adsorbed onto the NPs’ surface and form a protein corona layer, which mediates the interaction of NPs with cells. Here, we discovered the interaction of polystyrene (PS) NPs with protein fetal bovine serum (FBS) and altered cytotoxic effects. Mechanistically, prefabricated FBS protein corona mediated the relief of autophagic flux blockage, autophagosomes accumulation, and lysosomal damage in RAW264.7 cells caused by PS NPs. Using an individual fluorescent protein bovine serum albumin (BSA) as a corona surrogate, we demonstrated that coronal BSA remains, at least partially, on the surface of PS NPs during the initial stage of internalization and protects cell membrane from PS NPs-induced damage. However, along with the degradation of corona in lysosomes, reappearance of cytotoxicity was observed. Herein, we provided a proof of principle of the manipulation of corona on NPs’ toxicity and we expect the result will promote the further safety assessment of NPs.
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