Determination of Uric Acid Using TiO2 Nanoparticles Modified Glassy Carbon Electrode

In the present study, the electrochemical behavior of uric acid (UA) was evaluated using a titania nanoparticle (TiO2) coated glassy carbon electrode (GCE). TiO2 nanoparticles are synthesized and characterized using Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray Analysis (EDX) techniques. The electrochemical behavior of uric acid on both bare GCE and Titania coated GCE electrodes were studied through differential pulse voltammetry. Titania-coated GCE showed a higher current at the lower potential for the oxidation of uric acid when compared to bare GCE. The sensor's improved electrocatalytic activity was observed to detect uric acid in a 0.1 M phosphate buffer saline (PBS) solution at pH 7.0. A good linear relationship was observed between electrical response and the concentration of uric acid in the range of 1 to 9 μM. Under optimized experimental conditions, the limit of detection (LOD) was found as 0.764 μM. The sensor has expressed considerable sensitivity towards UA detection without interference and is successfully used to determine UA in human urine samples.